Journal: Molecular Therapy

Article Title: CD39 Produced from Human GMSCs Regulates the Balance of Osteoclasts and Osteoblasts through the Wnt/β-Catenin Pathway in Osteoporosis

doi: 10.1016/j.ymthe.2020.04.003

Figure Lengend Snippet: GMSCs Contribute to Bone Formation after Injection into Mice (A and B) A 50-μL Matrigel plug containing 1 × 10 5 GMSCs was implanted subcutaneously in immunocompromised mice. (A) Schematic of the transplantation model. (B) Representative gross look of subcutaneous tissue and micro-CT images illustrating organoids in a transplantation model. The quantification of bone volumes in subcutaneous tissues, n = 3. (C–E) 2 × 10 6 of GMSCs were fluorescently labeled with DiR and adoptively infused into C57BL/6 mice. (C) Schematic of the GMSC tracking model. (D) Representative digital IVIS images in different groups on 3 days after injection, n = 3. (E) Representative digital IVIS images and quantification of fluorescence intensity in different time points, n = 3. (F) GMSCs and osteoblast precursors (OBPs) cocultured (GMSCs to OBPs = 1:10) in the Transwell system; then induced osteogenesis followed with alizarin red staining. Representative gross look and image under microscope were shown under different conditions and the quantification by hexadecyl pyridinium chloride monohydrate in different treatment groups n = 3. The results represent three independent experiments (mean ± SEM), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by Mann-Whitney tests or t test or ANOVA test.

Article Snippet: A 50-μL Matrigel plug (Corning Matrigel Growth Factor Reduced Basement Membrane Matrix, 356231) containing 1 × 10 5 GMSCs was implanted subcutaneously, and the contralateral site was injected with a Matrigel plug as a control.

Techniques: Injection, Transplantation Assay, Micro-CT, Labeling, Fluorescence, Staining, Microscopy, MANN-WHITNEY